RNA Baser
 - The contig assembler. Many other contig assembly or chromatogram related toolsRNA Baser
 - a sequence assembly software
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chromatogram assembly
abi trace assembly
RNA Baser
 - a sequence assembly software (screenshot)

What is RNA Baser ?

 

 

RNA Baser represents an extension of DNA Baser, specialized in processing rRNA sequences. It is optimized for early integration of sequence and metadata. This way, the metadata will stay attached to the primary sequence information throughout the complete workflow of sequence analysis, until final submission.

 

- In addition, it is optimized for data exchange with standard non-commercial software used for rRNA sequence analysis and submission.

- It is a joint project of HeracleSoftware, Ribocon GmbH and Max Planck Institute for Marine Microbiology in Bremen, Germany



How are we helping you to analyze your rRNA sequences faster?

1. RNA Baser is performing for you all the tedious steps in sequence assembly:
- bad end trimming
- ambiguous bases correction
- vector removal


2. Interface designed with one purpose in mind: to minimize the user hands on time.
- synchronized display of the contig map, contig alignment and chromatograms
- fast navigation between one ambiguous bases: in more than 96% of the cases you don't need to make any correction. DNA Baser already has done it for you.

3. Assemble one clone library in one step:
Additionally to the single contig assembly mode, RNA Baser is offering the possibility to assemble hundreds of contigs in batch mode (link). Practically, due to the excellent quality check performed by RNA Baser (more than 96% of the errors are corrected), you can directly use the generated sequences for further analysis.

4. Fast integration of metadata in the sequence information:
- keep sequence and environmental parameters information together; in this way, you will always know were your sequence is coming from
- fast submission to the databases of all relevant data

 


Screenshot: batch assembly, single contig assembly, assemble to reference, contig map, bad end trimming, automatic error correction, chromatograms, rapid navigation through ambiguities, sequence assembly


With RNA Baser , you can:

  • Assemble or align to a reference sequence
  • Batch assemble or align in groups of sequences by name
  • Automatically end clip
  • Automatically trim vector sequences
  • Import sequences from ABI, SCF, FASTA and SEQ
  • View and edit sequence traces
  • Mark specific regions (like discrepancies, low-quality areas in chromatograms) with visible colors and quickly navigate to these regions
  • Convert between different file formats (ABI,SCF, FASTA, multi-Fasta...)
  • other features

 

RNA Baser price


The package is offered at a very affordable price, making it ideal for universities, research institutions, or private companies performing DNA sequencing. Discounts apply if you purchase more than one license.

 

 

ARB Silva

 

ARB Silva Opens in a new window is a comprehensive on-line resource for quality checked and aligned ribosomal RNA sequence data, free for academic use. SILVA provides comprehensive, quality checked and regularly updated databases of aligned small (16S/18S, SSU) and large subunit (23S/28S, LSU) ribosomal RNA (rRNA) sequences for all three domains of life (Bacteria, Archaea and Eukarya).

How to make a contig in 5 seconds

How to make a contig in 5 seconds

(click to enlarge)

 


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DNA chromatogram assembly
sequence assembly